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Image Search Results
Journal: Nature
Article Title: Clonally expanded CD8 T cells characterize Amyotrophic Lateral Sclerosis 4
doi: 10.1038/s41586-022-04844-5
Figure Lengend Snippet: a, Average latency to fall (sec) from an accelerating rotarod of WT and KI mice tested at 6 and 8 months of age (2 independent experiments, n=11 (WT 6 months and KI 8 months) and n= 13 (WT 8 months and KI 6 months) individual mice per group; mean +/− SEM). p-value calculated using the one-way Repeated Measures ANOVA (**p=0.0012) and Bonferroni’s post-test. (b,c) Bone marrow derived dendritic cells (BMDCs) from Setx WT and KI (b,c), and HET and KO (b) mice were infected with Citrobacter rodentium (multiplicity of infection=10, b) or stimulated with 5 μg/ml of cGAMP (c). Bar graphs show the relative induction of the indicated transcripts by RT-qPCR analysis at 2 hours post-infection or cGAMP stimulation in KO and KI cells normalized to their controls (2 independent experiments, n=4 mice/group, mean +/− SEM). No expression of the indicated genes was detected in uninfected cells. d, Concentration of the indicated cytokines measured by Luminex Assay in the serum of KI and WT (8–9 months old) animals (1 out of 2 independent experiments, n=6 (WT) and n=5 (KI) mice/group, mean +/− SEM). e, RT-qPCR analysis for the indicated inflammatory genes in the spinal cord of 10 months old WT and KI mice (Log2 relative expression against Actb, 2 independent experiments, n=5 mice/group, mean +/− SEM). f, Absolute numbers of indicated immune cell subsets in the spleen of 8–9 month old KI and WT mice. pDC: plasmacytoid dendritic cells; cDC: conventional dendritic cells. g, Expression of the activation marker CD86 in cDC and CD11b+ cells from the spleen of 8–9 months old WT and KI mice. (f,g) 2 independent experiments, n=4 mice/group, mean +/− SEM.
Article Snippet: After 5 days of culture, BMDCs were harvested and replated with MOI (multiplicity of infection) =10 of
Techniques: Derivative Assay, Infection, Quantitative RT-PCR, Expressing, Concentration Assay, Luminex, Activation Assay, Marker